Glial Derived Neurotrophic factor Human ELISA kit is an in-vitro enzyme link immunosorbent assay that measures the quantity of Human GDNF found in plasma and cells culture supernatants. This assay may not detect the human GDNF concentration in plasma. The assay uses an antibody that is specific for Human GDNF and coated on a 96 well plate.
The immobilized antibody is applied to the wells of the plates and the GDNF in the samples and standards are pipetted into them. After washing the wells, biotinylated anti Human GDNF antibody is added. After removing any unbound biotinylated antibodies, HRP-conjugated Streptavidin is pipetted into the wells. The amount of GDNF bound to the color will affect the development of the color in a GDNF ELISA equipment.
The Stop Solution changes the color to yellow and measures the intensity at 450 nm. Glial cell-line derived neurotrophic factors (GDNF), is a highly conserved member of the TGF Beta superfamily. The GDNF gene product can be processed into a disulphide linked homodimer.
GDNF repairs and protects dopamine-containing neuronal cells that are degenerate in Parkinson’s disease and motor neurons that die in amyotrophic lateral sclerosis. In the clinic, GDNF has been shown to be effective in treating Parkinson's disease. Neurological improvement has also been seen in the treatment of spinal cord injuries using GDNF.
The Human Glial – Derived Neurotrophic Factor Hu GDNF ELISA quantifies Hu GDNF levels in serum, plasma and cell culture media. This assay can only recognize natural and recombinant Hu GDNF. The microplate has wells that have been pre-coated with the target-specific antibody.
These wells can then be filled with samples, standards, and controls that bind to the captured (captured) antibody. The sandwich is formed by the addition of the second antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce a measurable signal.